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Figure 3: Survival of Bacillus subtilis B26 in the soil and plant tissues following the release of inoculum from PPI-alginate microcapsules. and timothy. (3A) Viable cell estimates (CFU) of B26 released from microcapsules incorporated into the soil before seeding (Pre-treatment). (3B) Viable cell estimates (CFU) of B26 released from microcapsules broadcasted on soil surface (Post-treatment). (3C) Viable cell estimates (CFU) of B26 roost and shoots of Brachypodium and timothy at harvest from the Pre-treatment. (3D) Viable cell estimates (CFU) of B26 in root and shoot of Brachypodium and timothy at harvest from the Posttreatment. (3E) DNA copy number of B26 in root and shoot of Brachypodium and timothy at harvest from the Pre-treatment. (3F) DNA copy number of B26 in root and shoot of Brachypodium and timothy at harvest from the Post- treatment. (3G) Phenotypic response of 56-days-old Brachypodium and timothy as a result of incorporation of loaded microcapsules or sterile microcapsules into the soil (Pre-treatment). (3H) Phenotypic response of 56-days-old Brachypodium and timothy as a result of broadcast of loaded microcapsules or sterile microcapsules into the soil (Post-treatment). *Represents a statistically significant difference p<0.05. |