Figure 5: High performance liquid chromatograms for B2’O fraction of O. africana, detection at 254 nm. Note: Chromatographic conditions: column, Tracer Extrasil ODS2, TR416059 (particle diameter, 5 μm; pore size, 30 nm; column size, 25 × 0.46 cm); eluent, methanol: water (80:20, v/v); flow rate, 1 ml/min; temperature, ambient.