Treatment V8 (mL.L-1) Vinaze (mL.L-1) Biomass (g.L-1) Conidia.mL-1 pH
T5.1 50 10 0.54a 4.65×107a 6.42bc
T5.2 50 15 0.78b 4.84×107a 6.34a
T5.3 50 20 0.35a 5.36×107a 6.34a
T5.4 100 10 1.50de 1.05×108b 6.63f
T5.5 100 15 1.07c 9.63×107b 6.52e
T5.6 100 20 1.62e 1.01×108b 6.39b
T5.7 150 10 0.83b 1.63×108d 6.46cd
T5.8 150 15 0.74b 1.46×108c 6.46cd
T5.9 150 20 1.32d 9.20×107b 6.42bcd
All treatments were inoculated with 1 × 106 conidia.mL-1 at 1% (v/v), growth conditions were maintained during 12 days after inoculation at 28 ± 2°C, 150 RPM and initial pH adjusted to 6.5 (NaOH 1N). Values indicate the means of four samples with three replicates each. Letters indicate significant differences by Tukey HSD (P ≤ 0.05).
Table 1: T. asperellum BP60 production of biomass, conidia concentration and pH response in liquid culture media containing different concentrations of V8 juice and vinaze.