Lines 1, 6 and 11 represents three subjects with normal genotype (198 bp fragment only), whereas lines 3, 4, 5, 7, 8, 9, 20, 12 and 13 show digested fragments (198 and 175 bp) from nine subjects. Lines 2 and 15 represents two homozygous carriers. The molecular weight marker used is 100 bp ladder (Newe England Biolab, The UK) in line 13.
On the figure 1 are presented genotype for C677T MTHFR polymorphism after subjecting to 4% agarose gel electrophoresis.
