|
Batch Adsorption |
Microfiltration |
Primary recovery step (per antigen) |
| Number of production runs to generate data |
4 |
4 |
| Capital cost |
$220,000 (TFF unit + batch adsorption tank) |
$200,000 (only TFF unit) |
| Resin cost |
$2,100 (per run)
Resin is suitable for 10 runs |
None |
| Cost of 0.2 um filters (2.4 m2 surface area used) |
$800 (per run)
Filters suitable for 10 runs |
$800 (per run)
Filters suitable for 10 runs |
| |
|
|
| Total buffer volume used for recovery for conditioning, cleaning, washing & elution (L) |
1685 ± 96 L |
1270 +50 L |
| % recovery (in comparison to fermentation yield) |
79 ± 18 % |
71 ± 19 % |
Purification step (per antigen) |
| Capital cost (Skid, column, TFF unit) |
$350,000 |
$437,500 ± 15,000 |
| Resin cost |
$3,475 ± 881 (per run)
Resins suitable for 10 runs |
$11,125 ± 581 (per run)
Resins suitable for 10 runs |
| Cost of 10K filters (2.4 m2 surface area used) |
$800 (per run)
Filters suitable for 10 runs |
$800 (per run)
Filters suitable for 10 runs |
| Final purity % |
98 ± 2 |
99 ± 1 |
| Total buffer volume used for purification for conditioning, cleaning, washing & elution (L) |
1268 ± 439 L |
1860 ± 256 L |
