Batch Adsorption

Microfiltration

Primary recovery step (per antigen)
Number of production runs to generate data 4 4
Capital cost $220,000 (TFF unit + batch adsorption tank) $200,000 (only TFF unit)
Resin cost $2,100 (per run) Resin is suitable for 10 runs None
Cost of 0.2 um filters (2.4 m2 surface area used) $800 (per run) Filters suitable for 10 runs $800 (per run) Filters suitable for 10 runs
     
Total buffer volume used for recovery for conditioning, cleaning, washing & elution (L) 1685 ± 96 L 1270 +50 L
% recovery (in comparison to fermentation yield) 79 ± 18 % 71 ± 19 %
Purification step (per antigen)
Capital cost (Skid, column, TFF unit) $350,000 $437,500 ± 15,000
Resin cost $3,475 ± 881 (per run) Resins suitable for 10 runs $11,125 ± 581 (per run) Resins suitable for 10 runs
Cost of 10K filters (2.4 m2 surface area used) $800 (per run) Filters suitable for 10 runs $800 (per run) Filters suitable for 10 runs
Final purity % 98 ± 2 99 ± 1
Total buffer volume used for purification for conditioning, cleaning, washing & elution (L) 1268 ± 439 L 1860 ± 256 L
Table 10: Summary of the comparison of process economics between batch adsorption and microfiltration at the production scale.