Run Date Juice (g/l)(X1) (NH4) H2PO4 (g/l)(X2) Seed size (% v/v) (X3)

Protein (g/100)(Y)

  Experimental Predicted
1 -1 -1 -1 42.910 42.843
2 1 -1 -1 45.875 46.003
3 -1 1 -1 41.775 41.811
4 1 1 -1 45.290 45.251
5 -1 -1 1 43.210 43.203
6 1 -1 1 43.670 43.587
7 -1 1 1 45.595 45.513
8 1 1 1 46.250 46.270
9 -1 0 0 44.441 44.551
10 1 0 0 46.405 46.463
11 0 -1 0 44.378 44.499
12 0 1 0 45.260 45.324
13 0 0 -1 44.515 44.549
14 0 0 1 44.088 44.962
15 0 0 0 45.720 45.507
16 0 0 0 45.505 45.507
17 0 0 0 45.530 45.507
18 0 0 0 45.501 45.507
19 0 0 0 45.640 45.507
20 0 0 0 45.500 45.507
Table 2: Face centered composite design matrix for evaluation of the three variables and experimental and predicted mycoprotein production.