S. No

Media sterilisation time (minutes)

Penicillin activity (1µ/mL)

Residual POAA (mg/mL)

1

25

35071

 4.21

2

30

45291

2.32

3

35

48195

1.05

4

40

48954

0.91

5

45

49057

0.14

6

50

30672

4.02

7

55

12876

6.21

Table 4: Penicillin activity and residual POAA content in various sterilization time intervals of 164 minutes fermentation media.