Figure 8: Recombinant production of GPI-proteins in eucaryotic cells. Soluble proteins, such as antibodies and protein drugs (e.g. insulin), are ectopically expressed as GPI-proteins in eucaryotic host cells in course of their plasmid-driven transcription, translation and co-translational translocation into the endoplasmic reticulum with concomitant coupling of the pre-fabricated GPI anchor to the carboxy- terminus of the protein domain and final transport to the outer surface of the plasma membrane along the classical secretory pathway. The GPI-proteins are then purified from the surface of the intact cells by detergent or “magnetic extraction”.