Factors Parameters
Forward Extraction  
AOT 20 mM
pH 7.0
Time 30 min
Temperature 4°C
Backward Extraction  
Salt concentration Optimum
pH Optimum
Isobutyl alcohol 15% v/v alcohol or 15% v/v distilled water
Time 1 hr
Temperature 4°C
No. of replicates=3 Total no. of runs= 6
Table 2: Effect of alcohol addition during backward extraction.