Run

Experimental factor

Result

A Glucose (g/L)

B Yeast-extract (g/L)

C KH2PO4 (g/L)

D MgSO4·7H2O (g/L)

Mycelia (g/L)

EPS (g/L)

1

1(20)

1(5)

1(1)

1(0.5)

5.25

0.71

2

1

2(6)

2(2)

2(1)

5.30

0.65

3

1

3(7)

3(3)

3(1.5)

5.24

0.75

4

2(30)

1

2

3

6.06

0.67

5

2

2

3

1

5.77

0.74

6

2

3

1

2

6.15

0.70

7

3(40)

1

3

2

5.39

0.74

8

3

2

1

3

5.41

0.87

9

3

3

2

1

5.88

0.84

The arrangements of column A, B, C, and D were decided by orthogonal design for 4 (factor)×9 (run number).
Table 2: Application of L9(34) orthogonal projects to the mycelial growth and EPS yield by Coriolus versiolor