Table 1

Primer name	Primer sequence	Application
ZDS DegF	5´ GGBGAACTTGATTTYCGVTT 3´	RT-PCR
ZDS DegR	5´ ATCACANGCWGCMACATATGCATC 3´	RT-PCR
ZDS-Fc1	5´ CCCCACCTAAGATGAAACCTGCCC 3´	5´ RACE-PCR
ZDS-Fc2	5´ GCGTGCCACCTTTGGAGATGAAC 3´	5´ RACE-PCR
ZDS-Fc3	5´ GCCCCAACTGGAAACCGGAAAT 3´	5´ RACE-PCR
ZDS-Fc4	5´ GCCCAGTTGTAAAGGCTCTCGTTGA 3´	3´ RACE-PCR
ZDS-Fc5	5´ GGTTCATCTCCAAAGGTGGCACGC 3´	3´ RACE-PCR
ZDS-EcoRI F	5´ CGGAATTCATGGCTTCTTGGGTTCTTTTCC 3´	PCR (full ORF)
ZDS-XhoI R	5´ CCGCTCGAGCTAAACAAGACTAAGCTTGTC 3´	PCR (full ORF)
	B=C, G, T; Y=C, T; V= A, C, G; N= A, T, C, G; W=A, T; M=C, A

Table 1: PCR primers used in this study, their oligonucleotide sequence is shown 5' to 3', and the purpose the primers were generated for. Note: F, forward; R, reverse; ORF, open reading frame.