Source DF SS MS F P Total 143 9836.37       Model Parts 3 587.33 195.78 395.34 0.001 EC 2 2888.51 1444.26 2916.43 0.001 HT 3 5083.29 1694.43 3421.61 0.001 Parts*EC 6 486.24 81.04 163.65 0.001 Parts*HT 9 486.34 54.04 109.12 0.001 EC*HT 6 128.11 21.35 43.12 0.001 Parts*EC*HT 18 129.00 7.17 14.47 0.001 Error 96 47.54 0.50     DF = Degree of freedom SS = Sum of square MS =Mean of square EC = Enzyme concentration HT = Hydrolysis time R2= 99.52 CV = 13.94%

Table 4: Analysis of variance for protein yield.