CHO HCP Standard (ng/ml) Colorimetric ELISA A450 Luminescent ELISA E425 Colorimetric ELISA S/N Luminescent ELISA S/N 0 0.075 ± 0.003 2461 ± 265.3 1 1 0.034 0.075 ± 0.004 3124 ± 140.6 0.993 1.269 0.102 0.082 ± 0.008 3389 ± 151.6 1.091 1.377 0.305 0.086 ± 0.008 3842 ± 351.9 1.139 1.561 0.914 0.093 ± 0.004 4924 ± 216.0 1.244 2.001 2.743 0.115 ± 0.004 7537 ± 432.9 1.533 3.063 8.23 0.153 ± 0.008 11179 ± 536.5 2.039 4.543 24.69 0.238 ± 0.018 18228 ± 335.9 3.17 7.407 74.07 0.313 ± 0.011 28313 ± 1656 4.17 11.51 222.2 0.419 ± 0.044 39576 ± 1913 5.577 16.08 666.7 0.502 ± 0.056 49976 ± 3293 6.683 20.31 2000 0.561 ± 0.034 64780 ± 3319 7.467 26.32 Mean     3.191 8.677 Stdev     2.418 8.736 P value (paired t-test on mean S/N, n=11)

Table 1: Comparison of the signal/noise (S/N) ratios of CHO HCP standards from colorimetric ELISA and luminescent ELISA performed in Corning Costar clear 96-well plate (n=3 for each standard concentration).