Table 2

CHO HCP standard (ng/ml)	Colorimetric ELISA		Luminescent ELISA
	% CV	% Rec	% CV	% Rec
2000	59.97	137.66	33.69	113.15^b
666.7^l	105.06	160.25	26.93^a	87.78^b
222.2^l	51.35	111.11^b	16.72^a	106.95^b
74.07^l,c	11.90^a	94.67^b	16.75^a	112.54^b
24.69^l,c	21.87^a	118.46^b	4.86^a	100.98^b
8.230^l,c	17.90^a	88.77^b	12.54^a	85.78^b
2.743^l,c	18.16^a	89.84^b	15.76^a	89.39^b
0.914^l	31.8	84.94^b	15.29^a	72.43^b
0.305	93.76	129.78^b	37.1	85.82^b
0.102	125.17	247.84	22.16^a	140.18
0.034	57.38	171.49	27.08^a	267.85
0	75.77		107.53

Table 2: Comparison of the precision (% CV) and accuracy (% Rec) of back-calculated concentration using 4-parameter fit for colorimetric and luminescent ELISA performed in Corning Costar clear 96-well plate.