Hydrolysis time (h)
1% LEP
5% LEP
D (1%)
D (5%)
0
30.5
30.5
0
0
4
31.2
31.5
0.7
1.0
8
34.4
41.3
3.9
10.8
24
28.7
33.7
--
3.2
Δ Variation between 4, 8 and 24 h and the zero time
Table 3:
Free acid production (μmol/ml) for different concentrations of LEP (with 4.7 U/mL of lipase activity) and hydrolysis times (Standard deviation less than 5%).