Hydrolysis time (h) 1% LEP 5% LEP D (1%) D (5%)
0 30.5 30.5 0 0
4 31.2 31.5 0.7 1.0
8 34.4 41.3 3.9 10.8
24 28.7 33.7 -- 3.2
Δ Variation between 4, 8 and 24 h and the zero time
Table 3: Free acid production (μmol/ml) for different concentrations of LEP (with 4.7 U/mL of lipase activity) and hydrolysis times (Standard deviation less than 5%).