Reagent/method Linear range μM/ L LOD μM/ L r2 R% RSD% λmax nm Ref.
Spectrophotometric              
0.1N HCl 5.0 - 25.0 10 <1 99.77 -- 211.3 [6]
Chemometric 50-100 0-20 0.998 95-105 1.18 220-250 [9]
RP-HPLC 1.5-7.5 0.05 0.999 99.3-100.6 0.067 240 [13]
RP-HPLC 0.2–3.5 0.079   97.3-99.9 1.36 235 [14]
RP-HPLC 5-30 0.66 0.999 97.2-99.6 < 2% 235 [16]
HPTLC 400-1400 ng/spot -- 0.998 100.16  ±  0.6 < 2% 235 [18]
HPTLC 300-600 ng/spot 8.8 ng  0.9866 98.5 - 101.2 <1.5% 220 [19]
HPLC-UV 0.005- 5.0 μg 2.0 ng  0.999 >99% <6.38 220 [22]
LC/MS/MS 10 - 10,000 pg 10 pg > 0.99 98.4-103.5 < 2% -- [23]
LC-MS/MS 0.5 to 250 ng -- 0.998 85 - 105 < 2% -- [24]
Capillary (CZE) 0.4-300 0.13 0.998 >99% < 2% -- [26]
Cyclic voltammetry 0.08 -1.0 mM 0.04 0.998 99.2-101.6 < 2% -- [29]
GC-MS 5-250 ng 2 ng 0.999 92 - 114 < 2% -- [12]
ISS Ref [33] Ref [34] [C.S] [C.S]
TpClPB- (PVC) (PMA) (ARS) (PTA) CMCPS
(DBP) (2-NPPE)
Parameter o-NPOE DOP Plastic Coated-Ag Coated-graphite CLO-ST
Slope (mV/decade) 61.7 59.3 55.97 57.57 58.03 ± 0.150 60.0 ± 0.5
Correlation coefficient (r) 0.9874 0.9993 0.9998 0.9999 0.9999 0.9999
Linear range (M) -- -- 1 x10-7-1x 10-2 1 x10-7-1x 10-2
LOD (M) 1.0 × 10-5 5.01x 10-8 4.1x10-8 5.0x10-8 3.5 x 10-8  
Working pH range 1.5 - 4.0 1.2-4.6 1.2-4.8      
Response time (s)     20 25 15 ≤ 8
Life span/days 77 84 25 30 40 Fresh surface
Accuracy (%) -- -- 99.09 ± 0.6 99.72 ± 0.24 99.28 ± 0.857 99.87 ± 0.177
Standard deviation -- -- 0.460 0.227 0.150 0.326
Robustness     99.48 ± 0.537 99.36 ± 0.337 99.51 ± 0.445 99.79 ± 0.23
Ruggedness     99.51 ± 0.430 99.26 ± 0.650 98.77 ± 0.199 99.83 ± 0.26
r2: Correlation coefficient R%: Mean recovery% C.S: Current Study
Table 7: Comparison between some of the published and the suggested methods for determination of CLO-ion.