Buffer type Buffer comparisons No of expts OPT buffer selection HM buffer selection Equilibration No buffer < GA-BK 3 of 3 GA-BK P-BK2 P-BK1 < GA-BK 4 of 6   P-BK2 = GA-BK 2 of 2 Resuspension P-P1 > GA-A1 3 of 4 P-P1 P-P1 Lysis P-P2 < GA-A2 3 of 4 GA-A2 P-P2 Neutralization P-P3-1 < GA-A3 3 of 4 GA-A3 P-P3-2 P-P3-2 < GA-A3 2 of 4 Pre-wash P-W1 = GA-W1 2 of 4 P-W1 P-W1 Wash P-W2-1 > GA-W2 3 of 4 P-W2-1 P-W2-1 P-W2-2 < GA-W2 2 of 4 Elution P-EB1 = GA-EB 2 of 4 P-EB2 P-EB2 P-EB2 > GA-EB 3 of 4 P-EB3 < GA-EB 3 of 3 [DNA] concentrations were measured in three separate readings using IMPLEN Nanophotometer P330. Independent T-test was used to determine the statistical significance of the differences between test buffers and generic brand A buffers. Differences in [DNA] were determined when p<0.05

Table 1: Comparison between the proprietary buffers and generic brand A miniprep kit buffers using DNA yields.