| Day |
Procedure |
| 0 |
Cell Revival in 125 mL spinner flask (50 mL working volume) |
| 1 |
First Medium Change |
| 2 |
Second Medium Change |
| 3 |
Subculture in 125 mL spinner flask (50 mL working volume) |
| 5 |
Scale upto 250 mL spinner flask (125 mL working volume) |
| 7 |
Scale upto 500 mL spinner flask (250 mL working volume) |
| 9 |
Scale upto 1000 mL spinner flask (500 mLworking volume) |
| 11 |
Inoculation into the bioreactor |
