| Run # 12 |
Run # 11 |
Run # 10 |
Run # 9 |
Run # 8 |
Run # 7 |
Run # 6 |
Run # 5 |
Run # 4 |
Run # 3 |
Run # 2 |
Run # 1 |
Parameter |
| 107.96 |
115.43 |
115.08 |
86.15 |
119.1 |
184.13 |
136.75 |
160.55 |
101.65 |
121 |
116.23 |
96.24 |
Culture Time (hrs) |
| 22.47 |
21.45 |
22.66 |
15.59 |
16.18 |
22.1 |
32.35 |
59.4 |
8.54 |
12.63 |
23.68 |
4.96 |
Final Antibody Concentration (mg/L) |
| 1.28 |
1.43 |
1.35 |
1.43 |
1.28 |
1.23 |
1.34 |
1.33 |
1.41 |
1.47 |
1.38 |
1.53 |
Final Working Volume (L) |
| 2.56 |
3.03 |
3.09 |
3.03 |
2.88 |
3.08 |
3.46 |
3.43 |
1.06 |
1.75 |
3.14 |
1.19 |
Peak Viable Cell Concentration (106 cells/mL) |
| 1.4 |
1.25 |
1.3 |
1.21 |
1.97 |
2.09 |
1.96 |
2.94 |
0.4 |
1.02 |
1.32 |
0.45 |
IVCD (x 108cells day/L) |
| 0.15 |
0.16 |
0.17 |
0.16 |
0.08 |
0.14 |
0.15 |
0.19 |
0.13 |
0.11 |
0.14 |
0.09 |
Average Specific Antibody Production Rate (mg/109cells per day) |
| 11.1 |
7.79 |
12.5 |
7.42 |
12.57 |
8.31 |
1.4 |
9.88 |
26.05 |
40.49 |
5.9 |
20.63 |
Average Specific Glucose Consumption Rate (mg/109 cells per day) |
| 3.67 |
3.17 |
2.69 |
3.02 |
3.36 |
1.95 |
2.42 |
2.17 |
12.87 |
3.04 |
3.02 |
10.79 |
Average Specific Glutamine Consumption Rate (mg/109 cells per day) |
| 20.23 |
20.31 |
17.3 |
19.34 |
20.58 |
5.37 |
14.67 |
6.66 |
79.67 |
32.84 |
18.89 |
38.23 |
Average Specific Lactate Production Rate (mg/109cells per day) |
| 0.78 |
0.59 |
0.5 |
0.56 |
0.39 |
0.24 |
0.55 |
0.22 |
1.63 |
0.54 |
0.61 |
0.98 |
Average Specific Ammonia Production Rate (mg/109 cells per day) |
| 35.1 |
33.8 |
32.1 |
35.55 |
49.1 |
25.75 |
35 |
26.6 |
38.4 |
40.3 |
36.2 |
27.3 |
Final lactate concentration (g/L) |
| 7.03 |
4.77 |
4.7 |
4.77 |
5.23 |
4.64 |
7.15 |
4.31 |
4.52 |
4.08 |
6.3 |
3.18 |
Final ammonia concentration (mmol/L) |
| 0.52 |
0.82 |
0.62 |
0.82 |
0.61 |
0.84 |
0.43 |
1.52 |
0.41 |
0.41 |
0.23 |
0.18 |
Viable cell concentration at harvest (106cells/mL) |
| 41.08 |
36.6 |
39.96 |
36.6 |
36.06 |
36.72 |
31.12 |
38.51 |
36.24 |
37.72 |
38.05 |
34.28 |
Viability at harvest (%) |
