Figure 1b: SDS-PAGE analysis was performed to analyze the DEAE fractions. Samples were prepared in loading buffer containing 60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue and run on a 10% polyacrylamide gel. Lane 1: Load sample, 40 μg, + βME; Lane 2: Flow through, 40 μg, +βME; Lane 3: Elution-1 peak sample, 40 μg, +βME; Lane 4: Ref. standard, 20 μg, +βME; Lane 5: Elution-2 peak sample, 40 μg, +βME; Lane 6: 0.5M NaCl wash, 40μg, +βME; Lane 7: 0.5M NaOH peak, 40 μg, +βME; C1-INH protein can be seen in elution-1 fraction, a less intense band whose electrophoretic mobility matched the reference standard in lane 4.