Figure 1c: Western blot analysis was performed to identify the fraction containing C1-INH protein. Samples were prepared in loading buffer containing 60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue and run on a 10% polyacrylamide gel and the gel is transferred on to a blot. Lane 1: DEAE load, 40 μg, +βME; Lane 2: Flow through, 40 μg, +βME; Lane 3: Elution-1 peak sample, 40 μg, +βME; Lane 4: Elution-2 peak sample, 40 μg, +βME; Lane 5: 0.5M NaCl wash, 40 μg, +βME; Lane 6: 0.5M NaOH peak, 40 μg, +βME; the protein of interest C1-INH was identified in load and elution-1 fraction.