Figure 2b: SDS-PAGE analysis was performed to analyze the Cellufine phenyl column fractions. Samples were prepared in loading buffer containing 60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue and run on a 10% polyacrylamide gel. Lane 1: Load sample, 40 μg, +βME; Lane 2: Flow through (Concentrated and diafiltered) 40 μg, +βME; Lane 3: Water wash peak 40 μg, +βME; Lane 4: NaOH peak, 40 μg, +βME.