Figure 3a: TMAE chromatography for the Cellufine phenyl flow through on a (1×10 cm, 20 ml) column. EQB: 25 mM sodium citrate containing 30mM NaCl, pH 6.0, conductivity 8 mS/cm.Wash-2: 25 mM sodium citrate pH: 7.0, Elution buffer: 25 mM sodium citrate containing 170mM NaCl, Conductivity 20 mS/cm, pH: 7.0.