Figure 3b: SDS-PAGE analysis was performed to analyze the fractions from TMAE column. Samples were prepared in loading buffer containing 60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue and run on a 10% polyacrylamide gel. Lane 1: Column load sample, 40 μg, +βME; Lane 2: Column flow through 40 μg, +βME; Lane 3: Elution peak sample; Lane 4: 0.5M NaCl elution peak 40 μg, +βME.