Figure 3: Time courses of the number of moles of hydrogen per culture volume (●), the concentrations of lactate (■), dry cell weight (♦) and unreacted fructose (○), and the number of moles of endogenous glucose per culture volume (▲). Cells were incubated under the dark anaerobic nitrate-free HEPES buffer solutions supplemented without (a) or with 25 mmol/L fructose (b). All experiments were carried out in duplicate.