Run no. Caffeine concentration (g/l) Cell loading (g/l) pH Theobromine produced (mg/l) Molar yield % Productivity (mg/l/h) Experimental Predicted Experimental Predicted Experimental Predicted 1 1 (≡3) -1 (≡4) 1 (≡9) 7.38 35.33 4.80 12.03 1.84 8.83 2 0 (≡2) 0 (≡8) 0 (≡8) 556.46 546.85 66.97 63.27 139.11 136.71 3 -1 (≡1) 1 (≡12) 1 (≡9) 149.79 157.95 48.40 50.31 37.44 39.49 4 0 (≡2) 0 (≡8) 0 (≡8) 556.46 546.85 66.97 63.27 139.11 136.71 5 -1 (≡1) -1 (≡4) -1 (≡7) 145.19 98.71 53.59 49.59 36.29 24.67 6 1 (≡3) 1 (≡12) -1 (≡7) 643.09 721.01 61.60 63.83 160.77 180.25 7 -1 (≡1) -1 (≡4) 1 (≡9) 6.55 -30.07 10.77 3.27 1.63 -7.53 8 0 (≡2) 0 (≡8) 0 (≡8) 546.26 546.85 60.06 63.27 136.56 136.71 9 -1 (≡1) 1 (≡12) -1 (≡7) 298.82 312.17 62.85 50.31 74.70 78.05 10 1 (≡3) -1 (≡4) -1 (≡7) 353.05 386.19 57.05 49.83 88.26 96.55 11 1 (≡3) 1 (≡12) 1 (≡9) 256.90 344.71 73.66 72.35 64.22 86.17 12 0 (≡2) 0 (≡8) 0 (≡8) 539.56 546.85 62.25 63.27 134.89 136.71 13 1.68  (≡3.68) 0 (≡8) 0 (≡8) 637.40 515.21 60.19 58.34 159.35 128.78 14 0 (≡2) 1.68 (≡14.73) 0 (≡8) 572.56 474.01 52.85 57.35 143.14 118.51 15 -1.68 (≡0.32) 0 (≡8) 0 (≡8) 67.09 116.85 27.77 39.63 16.77 29.19 16 0 (≡2) -1.68 (≡1.27) 0 (≡8) 8.45 34.82 0.47 6.07 2.11 8.70 17 0 (≡2) 0 (≡8) -1.68 (≡6.32) 487.05 453.57 58.96 70.42 121.76 113.40 18 0 (≡2) 0 (≡8) 1.68 (9.68≡) 67.92 29.30 40.16 38.67 16.98 7.32 19 0 (≡2) 0 (≡8) 0 (≡8) 530.29 546.85 60.06 63.27 132.57 136.71 20 0 (≡2) 0 (≡8) 0 (≡8) 548.26 546.85 63.56 63.27 137.06 136.71

Experimental response values are the average responses from three independent experiments

Table 2: Central Composite Design for optimization of reaction conditions for theobromine production by Pseudomonas sp.