Figure 7: Characterization of S. aurata astrocyte monolayer. Phase-contrast microscopy pictures showing the type-I and type II mature astrocytes within the monolayer in SaGliPs. (a) Primary neuroglia cultures in enriched medium differentiated in type-II astrocytes during monolayer formation after 2 weeks of culturing. (b) Neural elongated stem cells (NS) while generating neurons and astrocytes (stellate cells) in 1 week old cultures. (c) Primary neuroglia cultures in enriched medium differentiated in type-I mature astrocytes forming a monolayer after 3 weeks of culturing. (d) Monolayer of astrocyte cells with dense dendrite and neurite branching, and neuron bodies after 4 weeks of culturing (×200). (e) Monolayer of mature type-II astrocytes. (f) Morphology of a radial glia growing islet after 5 day. Scale bar = 50 μm.