Figure 8: Morphological characterization of S. aurata primary neuronal cells. (a, b) Phase contrast image showing fully differentiated communicating neurons with elongated neuritis overwhelming the astrocytes bed after 1 month of culture (×400). (c) Inverted contrast phase image showing early elongated neuritis growing over the astrocytes bed after 1 week of culture (×200). (d) Phase contrast image showing fully differentiated neurons with elongated neuritis overwhelming the astrocytes bed, 1 month of culture (×200). (e) Negative inverted phase contrast image showing intercellular communications (vesicles) between neurons 2 weeks (×400). (f) Enlarged area of (e) picture. Scale bar= 50 μm.