Figure 9: Oligodendrocyte lineage cell development and differentiation timing in neuroglial primary cultures from the brain of S. aurata. Phase-contrast microscopy images showing the lineage development of oligodendrocytes. (a) Glial-restricted precursor cells (GRPs); 2-day old cultures. (b, b’) Oligodendrocyte precursors (OPCs); 1-week old cultures. (c, c’) Enlargement of red area (d, e) Immature (iOL) and Mature Oligodendrocyte (mOL), 3 week old cultures.mOL showed a round cell body enriched of cytoplasmic granules and a dense filamentous meshwork in the peripheral areas often enwrapping other neuroglial processes. (f) Scheme of oligodendrocyte development. Scale bar = 50 μm (a, b, b’); 20 μm (c, c’, d, e)e.