Figure 10: Differentiation of oligodendrocyte lineage from S. aurata brain cultured cells. (a) Early stages of fiber engulfment by oligodendrocytes by contrast phase inverted image. Insert within (a) showing the CF aspect of a mature Oligodendrocyte (mOL): mOL showed a flattened round cell body enriched with cytoplasmic granules while forming a dense filamentous meshwork in the peripheral areas; 3-week old cultures. (b) Double immunolabeling with S100B antibody (red) and β-III tubulin (green); mOLs (red) were closely apposed to neuron fiber while enwrapping the neuronal axons (green). The S100B expression is essentially restricted to oligodendroglial cell lineage after 5 days of differentiation. (c) mOL showed enwrapping the long axon of a neuronal cell. (d, e) particulars of wrapped layers and prominent inner/outer tongue processes of mOLs; CF inverted images. Scale bar= 20 μm (a, b, d, e); 10 μm (a’, c).