No of uses of  DEAE-PGP complex Expressed peroxidase activity (%) Percent dye decolorization
DR19 495nm) DR19 and DB9 460nm)
1 89.7 91.5 82.2
2 78.8 87.4 80.6
3 69.7 74.6 72.7
4 58.4 69.8 65.4
5 50.7 64.9 61.5
6 42.4 60.6 59.4
7 36.3 58.9 51.8
8 29.7 51.4 43.2
9 18.9 33.6 28.5
10 15.5 21.4 19.6
Table 1: Re-usability of DEAE-immobilized PGP in decolourization of dye and dye mixture. Immobilized PGP was independently incubated with DR19 and mixture of dyes (DR19+DB9) (150 mL) at 40°C. Dye decolourization was determined after incubation period of 2 h. The immobilized enzyme was collected by centrifugation (3000 × g) and stored in assay buffer at 4°C overnight. The similar experiment was repeated 10 times. Each value represents the mean for three independent experiments performed in duplicate.