Figure 2: qPCR analysis of the effect of ICSD on pluripotent gene expression over 11 days of neuronal priming. Day 5 and day 11 columns represent nanog (A/D), oct4 (B/E) and sox2 (C/F) expression relative to day 0 expressions at the different ICSD in hESC and iPSC lines respectively. Expression of both nanog and oct4 was shown to decrease significantly in both hESC and hiPSC. Oct4 was shown to continually decrease over time whereas nanog remained stable. Sox2 expression was shown to increase slightly in both hESC and hiPSC. (*p< 0.05 & **p<0.01, Bars represent Mean ± SEM, n=3)