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Figure 4: Rheological properties behaviour of mature culture broths (E1 to E7) at selected times, expressed as function of the nematode concentration counted before shearing. Samples could correspond to day 20 for all experiments for the entomoparasitic nematodes Heterorhabditis bacteriophora growth in liquid systems in shake bottles (VL= 50 mL) or in bioreactor (VL= 4 L): (a) Flow behaviour index n [-], data produced with r2 = 0.7 for the best fit and (b) Flow consistency index K [Pa·sn], with a correspondent r2 = 0.85 for the best fit. Rheology data were estimated using a helical ribbon agitator fixed to a rheometer and applying mixer principles; concentration was estimated by direct count under light microscopy. |