Figure 4:1,4-β-D-glucan glucohydrolase homology model structure and active sites. Left side yellow : glucose ligand, Binding site residues : D88, F132, R146, K186, H187, R198, M230, F233, D265, W266, S405, M468. Nucleotide and deduced amino acid sequences were analyzed with CLC Free Workbench, Ver. 3.2.1 (CLC bio A/S, www.clcbio.com). Related sequences were obtained from database searches (SwissPort and GenBank). The biomolecular 3D structure of 1,4-β-D-glucan glucohydrolase was predicted with a deduced amino acid sequence as a homology model structure [8].