CloneID* DNAchange** Aminoacidchange*** Activity****       pH6.0, 50°C pH9.0, 50°C pH9.0, 60°C P1F10 45 C→T None ★★ ★ - P1C11 841  A → T 281   Thr → Ser ★ ★★ ★ P1H1 183   T → A None ★★★★★ ★★★★★ ★★★★ 503  T → A 168  Leu → Gln       534   T → C None       P2A2 386  C → T 123  Ala →Val ★★ ★★★ ★★ 177 A → G None       P2C1 102  C → T None ★ ★ - P2D3 800 G → A 267  Gly→ Ser ★★ ★★★ ★

*Clone ID means clone position among mutant library in 96-well plate. **Position and base in DNA sequence of xylanase krict PX1; Adenin (A), Timin (T), Sitosin (C), Guanin (G). ***Position and amino acid in natural xylanase and mutant; Threonine (Thr), Leucine (Leu), Serine (Ser), Glutamine (Gln), Alanine (Ala), Valine (Val), Glycine (Gly). ****The activity was arbitrarily expressed with results from 96-well micro-plate DNS assay.

Table 1: Changes of DNA, amino acid, and activity in selected mutants.