Figure 7: NIH-3T3 staining after 24 h of culture: nuclei appear in blue (Hoechst), actin filaments appear red (red-labeled phalloidin) and focal adhesion points appear green (green-labeled vinculin). A,B: Cells were stained immediately after incubation at 37°C. C,D: Cells were stained after a 20-min incubation at room temperature. A,C: Cells cultured on PNIPAM. B,D: Cells cultured on glass, which served as a control substrate. The close-up images (insets) are magnified 2.5 times. The scale bar is 20 μm.