Figure 7: 12% SDS PAGE of 48 hour fermented extract of B. subtilis (ATCC 6633) Lane 1, 48 hours fermented extract (4 μg), Lane 2, 0-30% fraction of ammonium sulphate precipitated fermented extract, lane 3, 30-80% fraction of ammonium sulphate precipitated fermented extract, Lane 4, Purified amylase, Lane M, Standard molecular weight markers (Sigma).