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Figure 5: Co-expression of PP1 and inhibitor-2. Top: Phosphatase activity in the IPTG induced bacterial extracts that contains genes for PP1 alone, PP1 and I-1 both and I-1 alone. Control samples are bacterial extracts without IPTG induction. Bottom: Western blot analysis in IPTG induced bacterial extracts: For I-1 in only I-1 containing bacteria and in co-transfected bacteria; For PP1 in only PP1 containing bacteria and co-transfected bacteria. |