| Trial # |
Independent variable |
Dependent variable |
| Feather (X1) |
Peptone
(X2) |
Yeast extract (X3) |
NH4Cl (X4) |
Incubation time(X5) |
Inoculum Size(X6) |
Casamino acids(X7) |
K2HPO4 (X8) |
Soluble proteins (mg/mL)(Y1) |
NH2-free amino groups (µmole leucine/mL)(Y2) |
| Exp.a |
Pred.b |
Exp.a |
Pred.b |
| 1 |
1 (1.0) |
-1 (0.0) |
1 (0.15) |
-1 (0.05) |
-1 (2) |
-1 (24) |
1 (0.2) |
1 (0.15) |
0.110 |
0.110 |
45.2 |
40.90 |
| 2 |
1 (1.0) |
1 (0.1) |
-1 (0.01) |
1 (0.15) |
-1 (2) |
-1 (24) |
-1 (0.0) |
1 (0.15) |
0.020 |
0.025 |
53.3 |
59.30 |
| 3 |
-1 (0.3) |
1 (0.1) |
1 (0.15) |
-1 (0.05 |
1 (6) |
-1 (24) |
-1 (0.0) |
-1 (0.03) |
0.095 |
0.104 |
41.9 |
39.91 |
| 4 |
1 (1.0 ) |
-1 (0.0) |
1 (0.15) |
1 (0.15) |
-1 (2) |
1 (48) |
-1 (0.0) |
-1 (0.03) |
0.128 |
0.132 |
82.0 |
77.54 |
| 5 |
1 (1.0 ) |
1 (0.1) |
-1 (0.01) |
1 (0.15) |
1 (6) |
-1 (24) |
1 (0.2) |
-1 (0.03) |
0.065 |
0.060 |
64.9 |
59.67 |
| 6 |
1 (1.0) |
1 (0.1) |
1 (0.15) |
-1 (0.05 |
1 (6) |
1 (48) |
-1 (0.0) |
1 (0.15) |
0.245 |
0.236 |
61.8 |
64.46 |
| 7 |
-1 (0.3) |
1 (0.1) |
1 (0.15) |
1 (0.15) |
-1 (2) |
1 (48) |
1 (0.2) |
-1 (0.03) |
0.094 |
0.090 |
35.4 |
39.83 |
| 8 |
-1 (0.3) |
-1 (0.0) |
1 (0.15) |
1 (0.15) |
1 (6) |
-1 (24) |
1 (0.2) |
1 (0.15) |
0.032 |
0.032 |
41.7 |
45.26 |
| 9 |
-1 (0.3) |
-1 (0.0) |
-1 (0.01) |
1 (0.15) |
1 (6) |
1 (48) |
-1 (0.0) |
1 (0.15) |
0.074 |
0.074 |
52.9 |
48.63 |
| 10 |
1 (1.0 ) |
-1 (0.0) |
-1 (0.01) |
-1 (0.05) |
1 (6) |
1 (48) |
1 (0.2) |
-1 (0.03) |
0.176 |
0.181 |
40.0 |
45.32 |
| 11 |
-1 (0.3) |
1 (0.1) |
-1 (0.01) |
-1 (0.05) |
-1 (2) |
1 (48) |
1 (0.2) |
1 (0.15) |
0.100 |
0.103 |
11.0 |
7.25 |
| 12 |
-1 (0.3) |
-1 (0.0) |
-1 (0.01) |
-1 (0.05) |
-1 (2) |
-1 (24) |
-1 (0.0) |
-1 (0.03) |
0.021 |
0.012 |
18.4 |
20.41 |
|
| • Values between in brackets are real values of independent variables.
• Real values of all independent variables except time and inoculum size are taken in terms of %W/V.
• Real values for the independent variable time are 24 hrs and 48 hrs.
• Real values for the independent variable inoculum size are taken in terms of %V/V providing that one mL of culture contained 5.0 × 106 CFU/mL.
• a: Experimental values b: Predicted values |
| Table 1: PBD and levels of eight independent variables in terms of coded and real values along with levels of corresponding experimental dependent variables. |
