Table 2

Trial #	Independentvariable			Dependentvariable
	Feather (X₁)	Inoculum size (X₆)	Yeast extract (X₃)	Soluble proteins (mg/mL) (Y₁)		NH₂-free amino groups (µmole leucine/mL)(Y₂)
	Feather (X₁)	Inoculum size (X₆)	Yeast extract (X₃)	Exp.a	Pred.b	Exp.a	Pred.b
1	-1 (0.5)	-1 (6.0)	-1 (0.15)	0.118	0.173	12.86	9.534
2	-1 (0.5)	-1 (6.0)	1 (0.45)	0.119	0.102	36.65	39.346
3	-1 (0.5)	1 (10.0)	-1 (0.15)	0.151	0.139	16.46	14.448
4	-1 (0.5)	1 (10.0)	1 (0.45)	0.165	0.178	28.12	37.410
5	1 (1.5)	-1 (6.0)	-1 (0.15)	0.266	0.269	62.12	53.954
6	1 (1.5)	-1 (6.0)	1 (0.45)	0.196	0.223	46.55	49.687
7	1 (1.5)	1 (10.0)	-1 (0.15)	0.234	0.267	80.55	78.979
8	1 (1.5)	1 (10.0)	1 (0.45)	0.421	0.382	49.43	53.881
9	-1.4 (0.3)	0 (8.0)	0 (0.3)	0.137	0.115	10.71	6.420
10	1.4 (1.7)	0 (8.0)	0 (0.3)	0.336	0.325	47.05	49.044
11	0(1.0)	-1.4 (5.2)	0 (0.3)	0.269	0.227	33.65	38.151
12	0 (1.0)	1.4 (10.8)	0 (0.3)	0.270	0.279	61.11	54.313
13	0 (1.0)	0 (8.0)	-1.4 (0.09)	0.269	0.213	42.25	52.450
14	0 (1.0)	0 (8.0)	1.4 (0.51)	0.231	0.244	70.30	55.750
15	0 (1.0)	0 (8.0)	0 (0.3)	0.259	0.279	32.48	40.400
16	0 (1.0)	0 (8.0)	0 (0.3)	0.284	0.279	46.50	40.400
17	0 (1.0)	0 (8.0)	0 (0.3)	0.271	0.279	32.48	40.400
18	0 (1.0)	0 (8.0)	0 (0.3)	0.284	0.279	46.50	40.400
19	0 (1.0)	0 (8.0)	0 (0.3)	0.271	0.279	40.00	40.400
20	0 (1.0)	0 (8.0)	0 (0.3)	0.259	0.279	40.00	40.400

• Values between in brackets are real values for the independent variables. • Real values for the independent variables feather and yeast extract are taken in terms of %w/v. • Real values for the independent variable inoculum size are taken in terms of %v/v providing that one ml of culture contained 5.0 × 10⁶ CFU/mL. • a: experimental values b: predicted values.

Table 2: CCD experiment with five levels of coded and real values for three factors along with the response.