Independent variable Coefficient Symbol Soluble proteins (1) (Y1) NH2 –free amino groups (2) (Y2)
  Estimate t-value P-value %confidence Estimate t-value P-value %confidence
B0 0.278816 19.28318 3.07E-09* 99.99 40.40009 11.442 4.56E-07* 99.99
Feather(X1) B1 0.075021 6.879394 4.3E-05* 99.99 15.22281 5.7165 0.000194* 99.99
Inoculum size(X61) B6 0.018603 1.705866 0.118844   5.77216 2.1675 0.055394 95.00
Yeast extract(X3) B3 0.010944 1.003568 0.339252   1.178679 0.4426 0.667458  
X12 B11 -0.02982 -2.35315 0.040423* 96.00 -6.46324 -2.088 0.063244  
X62 B66 -0.01298 -1.02437 0.329804   2.975538 0.9617 0.358881  
X32 B33 -0.02583 -2.01049 0.072116   6.990076 2.2284 0.049982 95.10
X1.X6 B16 0.020707 1.540874 0.154372   1.532481 0.4670 0.650503  
X1.X3 B13 0.006293 0.468305 0.649607   -8.51998 -2.596 0.026656 97.40
X3.X6 B36 0.040207 2.991949 0.013529* 98.65 -5.20752 -1.587 0.14361  
• Highlighted significant model terms are taken at P<0.05 for the soluble protein model. • Highlighted significant model terms are taken at P ≤ 0.06 for the NH2-free amino groups’ model. (1): R2=0.87 and adjusted R2=0.76 (2): R2=0.87 and adjusted R2=0.75
Table 6: Regression summary for full polynomial equation for end products of feather biodegradation upon using CCD.