Figure 6: The effects of volumetric concentration ratio (VCR) on both β-amylase activity recovery and specific enzyme activity in the retentate, The feed to this second UF was obtained with the following steps: (i) the first 3 kDa MWCO UF to the termination point of VCR ca. 7, (ii) the UF retentate as (i) salted-out using 1.2M (NH4)2SO4 and pH adjusted to 4, and (iii) the supernatant (i.e. this feed) obtained following the low-speed centrifugation to the slurry as (ii) and its pH adjusted back to 7. (■)Enzyme activity recovery in retentate; (○) Enzyme specific activity recovery in retentate.