Source of variation K. apiculatamF (R2= 0.98)* S. cerevisiae mc2 (R2= 0.97) O. oeni X2L (R2= 0.98) SS DF MS F-Stat SS DF MS F-Stat SS DF MS F-Stat Model 2.51 26 0.10 56.49* 3.12 26 0.12 32.99* 1.22 17 0.07 41.72* Culture (C) 0.51 2 0.26 149.95* 0.38 2 0.19 52.68* 0.06 1 0.06 33.18* Lyoprotectant (L) 1.3 8 0.16 95.13* 1.59 8 0.2 54.66* 0.67 8 0.08 48.73* C × L 0.7 16 0.04 25.49* 1.15 16 0.07 19.70* 0.49 8 0.06 35.78* Residuals 0.05 27 0.0017   0.1 27 0.0036   0.03 18 0.0017     *P< 0.0001. Sum of squares (SS), Degrees of freedom (DF), Mean squares (MS), F-Statistical (F-Stat)

Table 2: ANOVA test applied for all factors (culture and lyoprotectant solution) and their interaction on cell viability (Survival Factor to lyophilization, SFL) .