Source of variation SS DF MS F-stat P-value Model 84.12 863 0.10 31.90* <0.0001 Culture (C) 12.91 7 1.84 603.59* <0.0001 Temperature (T) 0.38 1 0.38 123.35* <0.0001 Lyoprotectant (L) 18.54 8 2.32 758.31* <0.0001 Time (t) 11.97 5 2.39 783.09* <0.0001 C × T 0.08 7 0.01 3.93* 0.0003 C × L 35.82 56 0.64 209.34* <0.0001 C × t 0.68 35 0.02 6.31* <0.0001 T × L 0.04 8 4.7 ×10-3 1.53* 0.143 T × t 0.50 5 0.10 32.54* <0.0001 L × T 0.21 40 0.01 1.74* 0.0036 C × T × L 0.27 56 4.8 ×10-3 1.59* 0.0049 C × T × t 0.15 35 4.2 ×10-3 1.38* 0.0717 C × L × t 1.09 280 3.9 ×10-3 1.28* 0.0047 T × L × t 0.09 40 2.3 ×10-3 0.76* 0.8593 C ×t× L 0.26 280 9.3 ×10-4 0.31* >0.9999 Residual 2.64 864 3.1 ×10-3     Total 86.76 1727       *P<0.0001. Sum of squares (SS), Degrees of freedom (DF), Mean squares (MS), F-Statistical (F-Stat)

Table 3: ANOVA test applied for all factors (culture, temperature, lyoprotectant, time) and their interaction on cell viability (Survival Factor to storage, SFS).