| S No |
Morphological Tests |
Observation |
Result |
| 1. |
Gram Staining |
Purple coloured rod shaped cells |
+ve bacilli |
| 2. |
Colony morphology |
Single celled white coloured, smooth, raised, circular with entire margin colonies on CMC agar plate |
Characteristics of colony resemble with growth of Bacillus sp. |
| S No |
Biochemical Tests |
Observation |
Result |
| 1. |
Catalase |
Slight Effervescence |
+ve |
| 2. |
Citrate utilization |
Blue colour |
+ve |
| 3. |
Indole production |
Cherry red colour |
+ve |
| 5. |
Starch hydrolysis |
Clear zone |
+ve |
| 6. |
Urease production |
No colour change |
-ve |
| 7. |
Methyl red |
No colour change |
-ve |
| 8. |
Vogesproskauer |
No colour change |
-ve |
| 9. |
Gelatin Hydrolysis |
Liquefaction |
+ve |
| 10. |
Sugar fermentation |
| (i) |
Sucrose fermentation |
Red to yellow colour Gas formation |
+ve |
| (ii) |
Lactose fermentation |
No change in colour Gas formation |
-ve |
| (iii) |
Glucose fermentation |
Red to yellow colour Gas formation |
+ve |
| (iv) |
Fructose fermentation |
Red to yellow colour Gas formation |
+ve |
| (v) |
Mannitol Fermentation |
Red to yellow colour Gas formation |
+ve |
