| Treatment No. |
Acid substrate |
Acid substrate loading every 12 hours |
Feed molar C/N ratio |
Culture media |
| 1 |
Acetic acid, propionic acid, butyric acid |
1.5 g/L total at 1:1:1 mass ratio |
70 every feeding |
SWW |
| 2 |
Acetic acid |
1.5 g/L |
70 every feeding |
SWW |
| 3 |
Acetic acid |
1.5 g/L |
70 at time 0 h |
SWW |
| 4 |
Acetic acid |
1.5 g/L |
70 at time 0 h |
SWW+sodium acetate every feeding to buffer acetic acid (equimolar, target pH=pKa=4.76) |
| 5 |
Acetic acid |
1.5 g/L |
70 at time 0 h |
SWW in 5X MOPS buffer set at pH 7.2 |
| 6 |
Acetic acid |
3 g/L |
140 at time 0 h |
SWW in 5X MOPS buffer set at pH 7.2 |
