Figure 5: Effect of the inoculated density of 3T3 cells on albumin secretion activity of hepatocytes. Micropatterned mono-culture (open circles); micropatterned co-culture inoculated with a 3T3 cell density of 1 × 105 cells (closed triangles); and micropatterned co-culture inoculated with a 3T3 cell density of 5 × 105 cells (closed squares). Error bars represent SD; *, p < 0.05 compared with the value of the mono-culture; #, p < 0.05 compared with the value of the co-culture at 1 × 105 3T3 cells.