Figure 1: Wolbachia are exclusively and unevenly distributed in lateral cords of adult male Brugia malayi. Sections A, B and C are cross sections. The top panel shows ISH results obtained with a 16S rRNA probe and the bottom panel shows ISH merged with DAPI staining. The color of ISH staining has been replaced by red to increase contrast before merging with DAPI staining. (A) Wolbachia are present in lateral cords on both sides of the worm; (B) no Wolbachia were seen in half of the lateral cord on one side; (C) Wolbachia are visible in one lateral cord. Abbreviations: i: intestine; lc: lateral cord; sp: developing sperm; dotted circles outline segments of lateral cords without Wolbachia; scale bar: 20 μm.