Figure 2: Wolbachia are present in oocytes and embryos and unevenly distributed in lateral cords of adult female Brugia malayi. Sections A, B and C are cross sections. The top panel shows ISH results obtained with a 16S rRNA probe, and the bottom panel shows ISH merged with DAPI staining. The color of ISH staining has been replaced by red to increase contrast before merging with DAPI staining. (A) Wolbachia are present in lateral cords on both sides of the worm and in pretzel stage embryos; (B) developing oocytes in ovaries and half of the lateral cord on one side contain Wolbachia; (C) Wolbachia are present in this section in morula stage embryos, but they are absent in the lateral cord on one side. Abbreviations: i: intestine; lc: lateral cord; ov: ovary; ut: uterus; dotted circles outline segments of lateral cords without Wolbachia; scale bar: 20 μm.