Figure 5: Expression of chemokines in NG97 cells line after meningococci infection. The Y axes show the log of Fold change in expression that was calculated according to the standard formula 2(En-Rn)-(Et-Rt), where En is the ct of the experimental gene in the normal sample, Rn is the ct of the reference gene in the normal sample, Et is the ct of the experimental gene in the treated sample and Rt is the ct of the reference gene in the treated sample. qRT-PCRs were repeated on three different biological replicates using as endogenous control de GAPDH gene and fold expression changes were averaged